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. 2014 Jan 24;13:11. doi: 10.1186/1476-4598-13-11

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Copyright © 2014 Hinrichsen et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nuclear and cytoplasmic SPTAN1 analysis. SPTAN1 was described to be involved in DNA repair processes. To determine cellular localization of SPTAN1, nuclear (N) and cytoplasmic (C) protein fractions of SPTAN1 overexpressing or corresponding untransfected HEK293 and HEK293T cells, cells were harvested and analyzed by Western blotting. The efficiency of the fractionation was verified by staining for Lamin B as a nuclear marker and gamma Adaptin as a cytoplasmic marker. In parallel, beta-Actin detection served for quantitative control.