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. 2014 Jan 24;13:11. doi: 10.1186/1476-4598-13-11

Figure 5.

Figure 5

Overexpression of SPTAN1 restores mobility of MLH1 deficient HCT116 mlh0-1 cells. Cell migration of (A) HCT116 mlh0-1, HCT116 mlh1-2, SPTAN1 overexpressing HCT116 mlh0-1 and siRNA SPTAN1 treated HCT116 mlh1-2 cells or (C) doxycycline treated MLH1 deficient HEK293T (HEK293T MLH1-) and untreated MLH1 proficient HEK293T (HEK293T MLH1+) cells were analyzed using an IncuCyte™ microscope. Distance of migration representative of three different experiments each done in quadruplicate was analyzed at different time points (0 h, 3 h, 6 h, 9 h and 15 h). (B and D) Representative corresponding images were taken from each cell line immediately after scratching the cultures (0 h) and 15 h later. Original Magnification 400×. Migration of MLH1 deficient HCT116 mlh0-1 could be restored to the level of MLH1 proficient HCT116 mlh1-2 after overexpression of SPTAN1 while mobility of HCT116 mlh1-2 treated with siRNA SPTAN1 was reduced to the level of HCT116 mlh0-1. Graphs indicate the results (mean ± S.D.) of at least three independent experiments.