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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Virology. 2013 Oct 29;448:196–209. doi: 10.1016/j.virol.2013.10.013

Figure 1. RRV infection induces lytic gene expression in a variety of cell lines from human and nonhuman Old World primate species.

Figure 1

(A) Rhesus primary fetal fibroblast (RPFF), (B) African green monkey kidney epithelial (Vero), and (C) human embryonic kidney epithelial (HEK293) cells were infected with RRV and evaluated three days post-infection for expression of the early lytic gene marker, the ORF59 DNA polymerase processivity factor, using rabbit anti-RV2 ORF59 antiserum (green), as described in Materials and Methods. The merged image of the ORF59 and Topro-3 (blue) nuclear staining is shown. Images were acquired by confocal microscopy and are shown as projections of the z-stack. The magnification scales are shown. Abundant RRV-infected ORF59-positive cell foci were detected in all three cell lines.