Figure 5. RRV Rta promoter activity is low in non-permissive cells and is activated by butyrate treatment.
Non-permissive AGS and HSG cells were transfected with the RRV Rta promoter construct, pGL2 R-Rta. Five hours post-transfection, butyrate was added to half of the cell cultures and luciferase activity was evaluated at 24 hours. Luciferase activity was normalized to the activity of pRL-CMV, as described in Figure 4. Error bars represent the standard deviation from triplicate wells. P values were calculated using the unpaired t test, two tailed.