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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Virology. 2013 Oct 29;448:196–209. doi: 10.1016/j.virol.2013.10.013

Figure 7. The conserved Sp1 element is critical for RRV Rta promoter activity.

Figure 7

(A) The Sp1 site in the critical RRV Rta promoter region was eliminated by site-directed mutagenesis in the full length pGL2 R-Rta construct. The conservation between the RRV and KSHV Sp1 sites are shown and the nucleotides matching the Sp1 consensus sequence (48) are highlighted. (B) Permissive Vero, HEK293 and HSG cells were transfected with either wild type pGL2 R-Rta or the Sp1 mutant and promoter activity was measured at 24 hours. pRL-CMV was co-transfected to control for transfection efficiency. The HSG cells were incubated with sodium butyrate 5 hours post transfection. Promoter activity, after normalization with pRL-CMV, is reported as percent of maximum activity. Mutagenesis of the RRV Sp1 site abrogated the RRV Rta promoter activity in all three permissive cell types. Error bars represent standard deviation from triplicate wells.