(a) Compared with an untreated 344SQ tumour, ZEB1 immunohistochemistry and (b) E-Cadherin immunofluorescent staining intensities following systemic miR-200b delivery. (c) Immunoblotting of tumour lystates for E-Cadherin (CDH1). (d) Relative tumour ZEB1 and ZEB2 expression levels following delivery. Data are averages ± s.e.m., n = 3. (e) Deep tumour delivery of Quasar 570-labelled (red) control miRNA in DOPC beyond the vasculature (CD31 staining—green, nuclear staining with Hoechst 33342—blue). Similar to a prior report24, ~80% of the fields had nanoparticle delivery, and within each field 35% of tumour cells contained signal. Scale bars, 100 μm (a + e); 50 μm (b).