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. Author manuscript; available in PMC: 2014 Jan 28.
Published in final edited form as: J Biol Chem. 2004 Oct 19;279(52):54140–54152. doi: 10.1074/jbc.M410493200

Fig. 7. 14-3-3τ regulates the expression of E2F1 target genes.

Fig. 7

A, HEK293 and U2OS cells were transfected with pSUPER or pSUPER-si14-3-3τ and were treated with adriamycin (5 μM) for 0, 6, or 12 h or NCS (300 ng/ml) for 0 or 5 h, respectively. RNA was extracted, and RT-PCR was performed using the primers specific for selected E2F1 target genes, p21, or glyceraldehyde-3-phosphate dehydrogenase. Mock RT-PCR represents a control reaction without the addition of RNA. B, a portion of cells in the experiments (A) was lysed, and cellular lysates were analyzed by Western blot analysis with indicated antibodies.