Skip to main content
. Author manuscript; available in PMC: 2014 Jan 28.
Published in final edited form as: J Biol Chem. 2004 Oct 19;279(52):54140–54152. doi: 10.1074/jbc.M410493200

Table I.

RT-PCR primer sequences and PCR conditions

Gene Sequences Annealing temperature Cycle number Size
°C bp
p73 5′-CATGGTCTCGGGGTCCCACT-3′
5′-CGTGAACTCCTCCTTGATGG-3′
62 50 471
Apaf-1 5′-AATGGACACCTTCTTGGACG-3′
5′-GCACTTCATCCTCATGAGCC-3′
56 45 331
Caspase 3 5′-TCGGTCTGGTACAGATGTCG-3′
5′-CATACAAGAAGTCGGCCTCC-3′
56 40 398
Caspase 7 5′-CAAAGCCACTGACTGAGATG-3′
5′-CAACCCAATGAATAAATGAT-3′
56 40 259
Caspase 8 5′-ACAGTGAAGATCTGGCCTCC-3′
5′-GCAGGTTCATGTCATCATCC-3′
56 50 375
Caspase 9 5′-GGCAGCTGATCATAGATCTGG-3′
5′-CTGCTTGCCTGTTAGTTCGC-3′
55 45 120
p107 5′-TGGTGTCGCAAATGATGCTGG-3′
5′-AGGAGCTGATCCAAATGCCTG-3′
55 40 362
Cyclin E 5′-CTCCAGGAAGAGGAAGGCAA-3′
5′-GTAAAAGGTCTCCCTGTGAAG-3′
55 25 421
TK-1 5′-ATGAGCTGCATTAACCTGCCCACT-3′
5′-ATGTGTGCAGAAGCTGCTGC-3′
60 35 204
p21 5′-CTCAGAGGAGGCGCCATG-3′
5′-GGGCGGATTAGGGCTTCC-3′
60 35 527
Glyceraldehyde-3-phosphate dehydrogenase 5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′
5′-AAATGAGCCCCAGCCTTCTCCATG-3′
55 40 325