Figure 1.

Rapamycin effectively blocks mTORC1 signaling in the brain. A, Dose-dependent suppression of P-S6 levels in the cortex and hippocampus after rapamycin injection. Rapamycin was applied to 2-month-old wild-type mice daily for 5 d. B, C, Rapamycin (Rapa) at 10 mg/kg effectively reduced P-S6 levels in both hippocampus and cortex of Pten mutant mice, as shown by Western blotting (B) and IHC (C). ctr, Control; mut, mutant. Control and mutant mice at 10 weeks of age were injected with either vehicle or 10 mg/kg rapamycin daily for 5 d. Scale bar, 100 μm.