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. 2014 Jan 21;5(1):e0001. doi: 10.5041/RMMJ.10135

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Copyright: © 2014 Somech and Etzioni.

This is an open-access article. All its content, except where otherwise noted, is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Neonatal Screening for SCID Using TREC Content.

The Guthrie card, used to collect heel-stick blood, is obtained routinely from every newborn. Quantitative PCR is performed from a 3-mm punch, and the number of TRECs is determined by comparison with standard serial dilutions of a plasmid containing the TREC sequence.