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. Author manuscript; available in PMC: 2014 Jan 28.
Published in final edited form as: Mol Cell Neurosci. 2009 Sep 4;42(4):382–390. doi: 10.1016/j.mcn.2009.08.010

Figure 2.

Figure 2

(A) ADP transiently stimulates Adenylyl Cyclase (AC) activity. BV2 cells were stimulated with 100 μM ADP for the time indicated and then cAMP concentration was measured with CatchPoint Cyclic-AMP fluorescent Assay kit. (B) ADP-induces AC activation through the Gi/o-coupled P2Y12 receptor. BV2 cells that were pretreated with inhibitors and cells were then lysed and subjected to cAMP assay. (C) FSK, OA, and H-89 have an inhibitory effect on ADP-induced membrane ruffling. Cells were pretreated with 10 μM FSK, 1 μM Okadaic acid (OA) or 30 μM H-89 for 20 min and then stimulated with 100 μM ADP. Lamella dynamics was then analyzed by kymographs. (D) FSK, OA, and H-89 have an inhibitory effect on ADP-induced chemotaxis of microglia cells. Transwell chamber membranes were coated with 3 μg/ml or 20 μg/ml fibronectin for 8 hr. Cells were then assayed for migration toward 100 μM ADP in the continued presence of H-89, FSK and OA. Averages of five independent experiments are shown.