Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Breast Cancer Res Treat. 2013 Nov 12;142(3):489–503. doi: 10.1007/s10549-013-2741-5

A) Representative photomicrographs (40X) of HGF staining in negative control (secondary only), 10%, 45% and 60% diet groups in normal (unaffected) mammary glands. Arrows indicate positive HGF staining in the stroma. B) Total HGF protein levels of the normal mammary glands were quantified using the Aperio ImageScope color deconvolution algorithm for n = 2 sections from 5 mice per diet group. * 10% vs. 60% (P = 0.011). C) c-Met mRNA expression was determined using qPCR analysis in normal mammary glands for n = 7. D) Representative photomicrographs (40X) of the IHC analysis of negative control and membrane localized c-Met staining in 10%, 45%, and 60% diet groups (arrows). E) Total c-Met protein levels in normal mammary glands were measured using the Aperio ImageScope membrane algorithm and quantified as above. * 10% vs. 45% (P = 0.04) and 10% vs. 60% (P = 0.001); ^ 45% vs. 60% (P = 0.025). F) Representative photomicrographs (40X) of the IHC analysis of negative control and pc-Met staining in 10%, 45%, and 60% diet groups. G) Total pc-Met protein levels in normal mammary glands were measured using the Aperio ImageScope color deconvolution algorithm and quantified as above. * 10% vs. 45% (P = 0.011) and 10% vs. 60% (P = 0.0001); ^ 45% vs. 60% (P = 0.0125).

A) Representative photomicrographs (40X) of HGF staining in negative control (secondary only), 10%, 45% and 60% diet groups in normal (unaffected) mammary glands. Arrows indicate positive HGF staining in the stroma. B) Total HGF protein levels of the normal mammary glands were quantified using the Aperio ImageScope color deconvolution algorithm for n = 2 sections from 5 mice per diet group. * 10% vs. 60% (P = 0.011). C) c-Met mRNA expression was determined using qPCR analysis in normal mammary glands for n = 7. D) Representative photomicrographs (40X) of the IHC analysis of negative control and membrane localized c-Met staining in 10%, 45%, and 60% diet groups (arrows). E) Total c-Met protein levels in normal mammary glands were measured using the Aperio ImageScope membrane algorithm and quantified as above. * 10% vs. 45% (P = 0.04) and 10% vs. 60% (P = 0.001); ^ 45% vs. 60% (P = 0.025). F) Representative photomicrographs (40X) of the IHC analysis of negative control and pc-Met staining in 10%, 45%, and 60% diet groups. G) Total pc-Met protein levels in normal mammary glands were measured using the Aperio ImageScope color deconvolution algorithm and quantified as above. * 10% vs. 45% (P = 0.011) and 10% vs. 60% (P = 0.0001); ^ 45% vs. 60% (P = 0.0125).