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. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: Breast Cancer Res Treat. 2013 Nov 12;142(3):489–503. doi: 10.1007/s10549-013-2741-5

A) Representative photomicrographs (40X) of the IHC analysis of tumors showing HGF staining in negative control and 10%, 45%, and 60% diet groups. B) HGF protein levels in tumors were quantified using the Aperio ImageScope color deconvolution algorithm for n = 2 sections from 5 mice per diet group. C) c-Met mRNA expression was determined using qPCR analysis in tumor tissues for n = 7. D) Representative photomicrographs (40X) of the IHC tumor analysis of membrane localized c-Met staining in negative controls and 10%, 45%, and 60% diet groups (arrows). E) Total c-Met protein levels in tumors were measured using the Aperio ImageScope membrane algorithm and quantified as above. F) Western immunoblot expression of c-Met in n=3 tumors per diet group and quantification (c-Met normalized to amido black staining loading control). G) Representative photomicrographs (40X) of the IHC tumor analysis of pc-Met staining in negative controls and 10%, 45%, and 60% diet groups. H) Total pc-Met protein levels in tumors were measured using the Aperio ImageScope color deconvolution algorithm and quantified as above.

A) Representative photomicrographs (40X) of the IHC analysis of tumors showing HGF staining in negative control and 10%, 45%, and 60% diet groups. B) HGF protein levels in tumors were quantified using the Aperio ImageScope color deconvolution algorithm for n = 2 sections from 5 mice per diet group. C) c-Met mRNA expression was determined using qPCR analysis in tumor tissues for n = 7. D) Representative photomicrographs (40X) of the IHC tumor analysis of membrane localized c-Met staining in negative controls and 10%, 45%, and 60% diet groups (arrows). E) Total c-Met protein levels in tumors were measured using the Aperio ImageScope membrane algorithm and quantified as above. F) Western immunoblot expression of c-Met in n=3 tumors per diet group and quantification (c-Met normalized to amido black staining loading control). G) Representative photomicrographs (40X) of the IHC tumor analysis of pc-Met staining in negative controls and 10%, 45%, and 60% diet groups. H) Total pc-Met protein levels in tumors were measured using the Aperio ImageScope color deconvolution algorithm and quantified as above.