Figure 2. Constitutive expression of miR-277 shortens lifespan. Comparison of survival curves under different food conditions. The panels on the left side represent a transgenic fly line established in the yw background, while the panels on the right represent an independent transgenic line established in the w¹¹¹⁸ background, then backcrossed into yw. The control flies for these experiments were w¹¹¹⁸ flies backcrossed into yw. Median lifespan and statistical significance were calculated in R! using the package “survival.” The different food compositions are indicated on top of each diagram. While differences due to genetic background are clearly visible, constitutive expression of miR-277 consistently shortens lifespan under all food conditions.