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. 2014 Jan 2;124(2):687–695. doi: 10.1172/JCI67313

Figure 3. CD8+ T cells are required for the efficacy of IR and anti–PD-L1 combination treatment.

Figure 3

(A) Tumor regression by combination treatment with anti–PD-L1 and IR was mediated by CD8+ T cells. Tumors received 12 Gy and mice were treated with anti–PD-L1, as described in Figure 2A. Starting from 1 day before IR, 250 μg of depletion antibodies against CD8+ T cells (clone 2.43) was injected i.p. every 3 days for a total of four times. *P < 0.05. (B) Combination therapy greatly enhanced the antigen-specific response of CD8+ T cells. TUBO tumors received 12 Gy, and mice were treated with anti–PD-L1 as described in Figure 2A. Nine days after IR, the draining LNs were removed and subjected to ELISPOT assays. **P < 0.01; ***P < 0.001. Representative data are shown from two (A) and three (B) experiments conducted with 5 to 6 (A) or 4 (B) mice per group.