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. 2014 Jan 9;124(2):656–669. doi: 10.1172/JCI70090

Figure 5. Regulation of the CXCR4 axis alters the frequency and amplitude of EPSCs of layer II/III pyramidal neurons.

Figure 5

(A) AMD3100 injection i.c.v. had no effect on sEPSC frequency or amplitude (sample data not shown). (B) Sample traces of mEPSCs recorded at –70 mV in the presence of tetrodotoxin (1 μM) and picrotoxin (100 μM) in layer II/III pyramidal neurons from vehicle- and AMD3100-exposed animals. Quantification revealed AMD3100 exposure significantly decreased mEPSC frequency, but not amplitude. (C) Incubation of prefrontal cortical slices with CXCL12 (20 nM, 3 hours) induced no change in sEPSC frequency, but significantly increased sEPSC amplitude (sample data not shown). (D) Sample traces of mEPSCs recorded at –70 mV in the presence of tetrodotoxin (1 μM) and picrotoxin (100 μM) in layer II/III pyramidal neurons from vehicle- and CXCL12-exposed slices. Quantification revealed CXCL12 exposure had no significant effect on mEPSC frequency or amplitude. *P < 0.04; #P < 0.03.