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. 2014 Jan 2;124(2):604–616. doi: 10.1172/JCI71017

Figure 1. Region-specific restoration of orexin receptor expression in Ox1r–/–Ox2r–/– mice.

Figure 1

Coronal brain sections prepared from Ox1r–/–Ox2r–/– mice with targeted injection of AAV-EF1α/OX1R::EGFP (B and D) or AAV-EF1α/OX2R::EGFP (A and C) were double-stained with anti-GFP (red) and the neuronal type–specific marker (green) antibodies TPH (A), TH (B), HDC (C), and ChAT (D). Regions denoted by white arrowheads are shown at higher magnification. Schematics show the spread of OX1R::EGFP or OX2R::EGFP expression. Mean numbers of EGFP+ cells are shown by green or red circles, indicative of the absence or presence, respectively, of the neuronal type–specific marker. For bilaterally injected areas, only 1 side was shown. 3v, third ventricle; 4v, fourth ventricle, Aq, aqueduct; SNR, substantia nigra pars reticulata; VMH, ventromedial hypothalamus. Scale bars: 100 μm.