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. 2014 Jan 2;124(2):604–616. doi: 10.1172/JCI71017

Figure 5. Restoration of orexin receptor expression selectively in DR serotonergic and LC noradrenergic neurons suppresses cataplexy-like episodes and ameliorates fragmentation of wakefulness, respectively.

Figure 5

(A–C) Control virus–injected Ox1r–/–Ox2r–/– (as in Figure 2; n = 16), Ox1r–/–Ox2r–/–+5HT-OX2R (n = 6), Ox1r–/–Ox2r–/–+NA-OX1R (n = 5), Ox2r–/– (n = 5), and WT (n = 4) mice were recorded. Mean values of 3 nights’ recordings are shown. Data from Figure 2 are included for comparison. (A) Number of and time spent in cataplexy-like episodes. (B) REM sleep latency and time spent in REM sleep. (C) Duration and number of wakefulness episodes during the dark phase. *P < 0.05, **P < 0.01 vs. Ox1r–/–Ox2r–/– control; #P < 0.05, ##P < 0.01 vs. WT. (D) Time-weighted frequency histograms of wakefulness duration in control Ox1r–/–Ox2r–/– (n = 16), Ox1r–/–Ox2r–/–+LC-OX1R (n = 8), Ox1r–/–Ox2r–/–+NA-OX1R (n = 5), Ox2r–/– (n = 5), and WT (n = 4) mice, showing the wakefulness that occurred in episodes of each length as a percentage of total wakefulness in the dark phase. **P < 0.001 vs. Ox1r–/–Ox2r–/– control; ##P < 0.001 vs. WT; ++P < 0.001 vs. Ox2r–/–, 2-way repeated-measures ANOVA. Values are mean ± SEM.