Figure 3. PF4 maintains normal Th differentiation.

CD4⁺ T cells were isolated from WT and Pf4^–/– mice, and qRT-PCR was performed. Pf4^–/– CD4⁺ T cells had (A) decreased expression of Th1, (B) increased expression of Th17, and (C) decreased expression of negative regulators of Th17 differentiation (n = 4; mean ± SD; *P < 0.01 vs. WT). (D) Pf4^–/– mouse inguinal lymph nodes also had increased Th17 cells (n = 4; mean ± SD. *P < 0.01 vs. WT).