Figure 8. OTX2 binds to promoters of rod genes, including Nrl, in WT and Crx^–/– retinas but not in Crx^Rip/Rip retinas.

(A) ChIP-qPCR with anti-OTX2 antibody from P21 WT, Crx^Rip/Rip, and Crx^–/– retinas. Normal IgG was used as control. Fold enrichment represents the fold change of qPCR amplification signals for the different genes tested between OTX2 ChIP DNA and IgG control ChIP DNA. (B) Representative images of P21 Crx^Rip/+ mouse retinas electroporated at P0 with RHO-DsRed (red) and either OTX2-GFP or Ub-GFP (green; mock). Scale bar: 40 μm.