Figure 1. DOX regulates mitochondrial iron levels.

DOX levels in the (A) mitochondrial and (B) cytosolic compartments of NRCMs after 16 hours of treatment with 10 μM DOX. (C) Mitochondrial iron levels, as assessed by colorimetric measurement of mitochondrial nonheme iron, in NRCMs that had been treated with DOX (10 μM) or a vehicle control for 16 hours (n = 5). (D) Mitochondrial iron levels, as assessed by measurement of ⁵⁵Fe, in DOX-treated NRCMs (10 μM for 16 hours, n = 4). Data are presented as mean ± SEM. *P < 0.05.