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. 2013 Sep 25;25(1):1–13. doi: 10.1089/hgtb.2013.014

FIG. 4.

FIG. 4.

Tamoxifen-induced ATOH1-ER-DSRED fusion protein activation of the 3′ Atoh1 promoter. (A) HEK cells were stably transfected with a 3′Atoh1enhancer/promoter.luciferase construct, transiently transfected with either the Atoh1-ER-DsRed or DsRed-ER control construct, incubated for 72 hr in increasing doses of tamoxifen, and then lysed and subjected to luciferase assay. (B) HEK cells transfected with the DsRed-ER construct exhibited a tamoxifen-dependent nuclear translocation of DsRed fluorescence similar to the Atoh1-ER-DsRed construct shown in Fig. 2. (C) Luciferase assays indicated that HEK cells transfected with DsRed-ER control constructs exhibited no significant change in 3′Atoh1enhancer/promoter.luciferase activity. HEK cells transfected with Atoh1-ER-DsRed exhibited a significant (*p<0.05) increase in luciferase activity in the presence of tamoxifen, indicating a tamoxifen-dependent binding of the ATOH1-ER-DSRED fusion protein to the 3′ Atoh1 promoter region.