FIG. 8.

Electroporation of the Atoh1-ER-DsRed construct into cultured organs of Corti. Organs of Corti were isolated from neonatal pups that express a nuclear tagged GFP under control of the 3′ Atoh1 promoter, electroporated with the Atoh1-ER-DsRed construct, and cultured for an additional 3–5 days. (A–C) Electroporated cells exhibited diffuse cytoplasmic labeling in organs of Corti cultured in the absence of tamoxifen. (D–G) Organs of Corti cultured in 1 nM tamoxifen exhibited punctate signaling that co-localized with punctate GFP signaling. (H) Immunolabeling of the flag-tag sequence of the ATOH1-ER-DSRED fusion protein (red) colabels for MYOSIN 7a immunostaining (green).