FIG. 2.

Fluorescence microscope images of the control (laser-untreated/vector-uninjected and laser-treated/vector-uninjected) and scAAV type 2, 5, and 8 CMV EGFP vector-transduced fundi. The vector doses were 1.27×10⁷, 3.38×10⁷, and 3.65×10⁷ VG/eye, respectively, and the exposure time was 1500 msec. The optic nerve head is positioned at the center of the each image. The fundi of the control demonstrated only scant autoflurescence around the optic nerve head, regardless of the laser pretreatment (A and E). In the laser-untreated/vector-injected group, AAV2 vector resulted in scattered fluorescence spots around the entire fundus and fluorescence in retinal nerve fiber/ganglion cell layer (B), while AAV 5 and 8 vector treated eyes showed occasional fluorescent spots around the optic nerve head (C and D). Laser pretreatment produced increased transduction around the laser sites in all capsid types (F–H). Dark, hypofluorescent areas around laser burn site were due to RPE cell proliferation (G and H, arrowheads). Asterisk (*), laser burn sites. Scale bars: 200 μm.