Fig. 6.

Total S-nitrosylated proteins and S-nitrosylated APX in leaves of pea plants under salinity stress conditions. (A) Detection of total S-nitrosylated proteins from leaves of pea plant controls and those exposed to 150mM NaCl. S-nitrosylated proteins were separated under non-reducing conditions by 12% SDS–PAGE and blotted onto a PVDF membrane. Biotinylated proteins were detected using anti-biotin antibodies as described in the Materials and methods. (B) Immunoblot of total S-nitrosylated proteins of leaves of pea plant controls and those exposed to 150mM NaCl probed with a polyclonal antibody against cucumber APX (dilution 1:3,000). A 5 μg aliquot of protein was used per lane.