Table 2.

Densitometry analysis of GUS staining in transactivation assays for MYB–promoter interactions

TF vector	GSa	PgDHS2pro			Pg4CLpro
		No TFb	TFc	Ratio TF/no TF	No TF	TF	Ratio TF/no TF
PgMYB8	Spot	24	223	9.29	26	308	11.85
	Density	3254	34 590	10.63	3574	45 939	12.85
PgMYB15	Spot	24	2	0.08	26	0	0.00
	Density	2291	266	0.12	3574	0	0.00
PtMYB1	Spot	113	373	3.30	16	85	5.31
	Density	17 298	63 295	3.66	2424	14 279	5.89
PtMYB8	Spot	72	191	2.65	28	156	5.57
	Density	9672	31 545	3.26	3347	18 595	5.56
PtMYB14	Spot	168	36	0.21	75	11	0.15
	Density	24347	4858	0.20	11258	1397	0.12

^a GUS staining (GS), number of blue spots or total density across all four filters (replicates) (see Materials and methods).

^b No TF, empty vector control.

^c TF, MYB expression vector.