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. 2013 Dec 9;65(2):621–639. doi: 10.1093/jxb/ert412

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — GUS staining patterns in AIF:GUS transgenic Arabidopsis. (A) GUS activity was specifically detected in the anthers of AIF:GUS floral buds (stages 7–11) and was absent in the young floral buds (yfb) before stages 5–6. The number indicates the stage of flower development. (B) Close-up of young floral buds at stages 3–9. GUS activity started to be detected in anthers (arrowed) of floral buds at stage 7 and was strongly expressed at stages 8–9. GUS was absent in the young floral buds before stages 5–6. (C) The corresponding young floral buds at stages 3–6 in (B) are indicated by dashed circles. (D) Close-up of a floral bud at stage 8. Strong GUS activity was detected only in anthers (an) and in the upper parts of filaments (arrowed). (E and F) Close-up of a floral bud (E) and anther (F) at stage 10. Strong GUS activity was detected in anthers (an) and pollen (arrowed). (G) GUS staining was weak in anthers (an) and pollen of an AIF:GUS mature flower at stage 12. c, carpel; s, sepal; p, petal; f, filament.