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. 2014 Jan 27;65(2):709–723. doi: 10.1093/jxb/ert432

Fig. 7.

Fig. 7.

Specific interaction of POTH1 and four KNOTTED1-type proteins of tobacco with nine BEL1-like proteins, BEL5, -30, -13, -6, -31, -32, -33, -34 and -35, of potato using a β-galactosidase assay to assess the strength of interaction. The new potato BELs were cloned into the pACT-AD vector and the KNOTTED1-types were expressed in pBridge. BEL5/POTH1-BD was used as a reference and the new BEL proteins in the pACT-AD vector transformed into yeast are shown as negative controls. LacZ induction in the yeast strain pJ69-2A was assayed in transformed yeast cultures using a quantitative yeast β-galactosidase assay method. Standard errors of the means of three replicate samples are shown for each combination.