Table 2.
Eight target genes of StBEL5The TGAC core motifs running 5′→3′ are in bold letters. Linker sequence between the motifs is shown in lower-case letters. The location of the motif is designated upstream from either the transcription (TSS) or the translation (AUG) start site.
| Gene | Motif a | Orientationb | Location of motif (nt upstream) | RNA levels regulated by StBEL5 | Binding confirmed by EMSAc | Reference |
|---|---|---|---|---|---|---|
| StBEL5 | GTCAAtgcTTGAC | HtH | 820 (TSS) | Yes | Yes | Lin et al., 2013 |
| StBEL6 | TTGACaGTCA | TtT | 520 (TSS) | Yes | Yes | Figs 8 and 9 |
| StBEL22 | GTCAcaaTTGAC | HtH | 1471 (TSS) | Yes | Yes | Figs 8 and 9 |
| StBEL34 | TTGACggTGAC | TtH (+) | 1459 (TSS) | Yes | Yes | Figs 8 and 9 |
| StGA20ox1 | TTGACTTGAC | TtH (+) | 700 (TSS) | Yes | Yes | Chen et al., 2004 |
| StGA2ox1 | TTGACaaGTCA | TtT | 1768 (AUG) | Yes | Yes | Lin et al., 2013 |
| StIPT | TTGACaaGTCA | TtT | 1408 (AUG) | Yes | Yes | Hannapel et al., 2013 |
| YUCCA1a | TTGACcttaTTGAC | TtH (+) | 641 (AUG) | Yes | Yes | Lin et al., 2013 |
a The criteria for these motifs was the inclusion of at least one TTGAC and one TGAC on either strand of the DNA with a linker sequence of no more than four nt.
b Three 5′→3′ orientations were observed: head-to-head (HtH), tail-to-tail (TtT), or tail-to-head on the plus strand (TtH(+)). No double motifs aligned tail-to-head on the (–) strand were identified in this initial screen.
c Verified binding to the BEL5/POTH1 complex via EMSA. Stronger binding with the BEL5/POTH1 complex was observed than with either protein alone.