. 2013 Dec 27;65(2):655–671. doi: 10.1093/jxb/ert435

Table 1.

Identification, functional categorization, and quantitative analysis of the differentially displayed proteins

Spot Exp. name^a	Protein score	Sequence coverage (%)	Peptide matches	Exp./Theo.		Protein hits	Protein identification	Changes in spot intensity^b
Spot Exp. name^a	Protein score	Sequence coverage (%)	Peptide matches	Mol. wt (kDa)	pI	Protein hits	Protein identification	Changes in spot intensity^b
Biosynthesis
OsUP8	290	22	10	31.7/56.9	6.21/8.93	gi\|218165	Pre-pro-glutelin
OsUP12	610	26	11	40.3/56.8	7.01/9.17	gi\|169791	Glutelin
OsUP13	138	16	6	53.6/54.3	7.79/9.30	gi\|218190412	Glutelin
OsUP14	283	31	9	23.9/57.4	6.71/8.96	gi\|115445979	Glutelin
OsUP16	151	14	7	50.4/38.8	5.96/5.73	gi\|22748337	Putative gln1_oryza glutamine synthetase isozyme
OsUP18	484	27	10	36.2/50.7	6.26/8.74	gi\|213876598	Gt3, partial
OsDP2	394	9	9	119.3/114.1	6.52/6.35	gi\|218194315	Hypothetical protein OsI_14800 (pullulanase)
OsDP4	282	23	13	146.7/103.6	5.47/5.98	gi\|115463815	Orthophosphate dikinase
OsDP6	193	5	7	121.1/69.5	6.49/6.61	gi\|115470493	Starch debranching enzyme
OsDP8	178	28	11	93.4/67.0	6.92/8.16	gi\|19911776	Granule-bound starch synthase
Energy metabolism
OsUP5	355	40	13	35.9/27.4	5.41/5.39	gi\|125528336	Dihydroxyacetone phosphate and d-glyceraldehyde-3-phosphate dehydrogenase
OsUP19	147	18	6	44.2/50.1	6.89/5.44	gi\|115463933	Putative GDP dissociation inhibitor
OsDP3	353	36	13	109.4/58.3	5.91/5.48	gi\|115438749	ADP-glucose pyrophosphorylase large subunit
OsDP5	596	41	14	78.4/51.8	5.45/5.43	gi\|115480571	UDP-glucose pyrophosphorylase
Oxidation
OsUP3	490	41	10	34.2/27.3	5.43/5.42	gi\|115452337	l-Ascorbate peroxidase
OsUP4	220	35	8	20.7/35.6	5.89/8.22	gi\|115465579	Putative malate dehydrogenase
OsUP6	336	65	9	10.4/13.3	5.27/5.16	gi\|115470941	Chain A, solution structure of thioredoxin type H from Oryza sativa
OsUP7	265	53	8	127.8/15.2	6.50/5.92	gi\|115473931	Copper/zinc superoxide dismutase
OsUP10	459	50	12	33.1/27.3	5.54/5.42	gi\|115452337	Ascorbate peroxidase and cytochrome c peroxidase
OsUP11	318	32	10	44.2/35.6	6.89/8.22	gi\|115465579	Putative r40c1 protein, ricin-type beta-trefoil lectin domain-like protein
OsUP15	161	23	5	42.5/40.0	5.56/7.82	gi\|115482392	Putative conserved protein
Heat shock
OsUP1	276	29	9	18.2/18.1	5.51/4.57	gi\|115463081	18.1kDa heat shock protein
OsUP2	341	31	9	18.0/17.9	5.91/5.23	gi\|18031726	17.9kDa heat shock protein
OsDP1	101	19	12	133.4/65.2	6.39/6.03	gi\|115447567	Tetratricopeptide repeat domain protein (similar to heat shock protein STI).
Transcript regulation
OsUP9	167	27	5	42.5/28.5	5.40/5.45	gi\|115464745	Basic/helix–loop–helix (bHLH) transcription factors

^a Spot experiment name corresponds to the spots in Figs 2 and 3. OsUP and OsDP indicate up- and down-regulated proteins, respectively.

^b Relative expression graphs of the protein spots after high temperature treatment in heat-sensitive and heat-tolerant rice lines. Mol. wt and pI indicate molecular weight and isoelectric point, respectively. Grey bars indicates heat-sensitive rice, and black bars indicates heat-tolerant rice. Spot volumes were analysed by Progenesis software. The fold change of up-regulated protein spot volumes was calculated by treatment/control, whereas the change fold of down-regulated protein spot volumes was calculated by control/treatment. From left to right, each bar indicates the fold change in protein spot volumes compared with control after exposure to high temperature for 1, 3, and 5 d. Values are presented as means ±SE. Error bars are from three spots in three independent gels.