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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Nov 26;449:62–69. doi: 10.1016/j.virol.2013.11.014

FIG. 1.

FIG. 1

RSV repression of GR-induced genes is not mediated by a soluble secreted factor. Conditioned media from mock-infected cells (CM) (open bar), RSV-infected cells (RSV CM) (solid bar) or UV-irradiated conditioned media from RSV-infected cells (UV-RSV CM) (hatched bar) was added to uninfected A549 cells. After 5 hours vehicle or dexamethasone was added such that the final concentration was 100 nM and cells were incubated for a further 5 h. A. IL-8 mRNA was detected in vehicle only treated cells by real-time PCR, normalized to GAPDH and CAP-1 and compared to untreated cells. Dexamethasone-induced GILZ (B) and MKP-1 (C) mRNA was determined by real-time PCR and normalized to GAPDH and CAP-1. Means and SD are shown (n= 5). Significance was tested using a 1-way ANOVA and Dunnett post hoc test (*** depicts p<0.001).