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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Nov 26;449:62–69. doi: 10.1016/j.virol.2013.11.014

FIG 2.

FIG 2

Knock-down of MyD88 or RIG-I does not reverse RSV repression of dexamethasone-induced GILZ mRNA. A549 cells were transfected with control siRNA or siRNA against MyD88 or RIG-I. 48 h later they were inoculated with rgRSV (MOI=3) (solid bar) or mock-infected with HeLa-conditioned media (open bar) for 1 h. The media was aspirated and cells treated with vehicle or 100 nM dexamethasone for 5 h. Dexamethasone-induced GILZ mRNA was determined by real-time PCR and normalized to GAPDH and CAP-1 (A). siRNA knockdown was confirmed by real-time PCR (B & C). Means and SD are shown (n= 3). Significance was tested using a 1-way ANOVA and Tukey post hoc test (** depicts p = 0.01-0.001; *** depicts p<0.001).