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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Nov 26;449:62–69. doi: 10.1016/j.virol.2013.11.014

FIG 4.

FIG 4

Over-expression of NS1 represses glucocorticoid-induced MMTVLuc activity. Cos7 cells were transfected with hGR, the GR-responsive MMTVLuc promoter; a constitutively active control renilla vector, and either a HA-(A and C) or FLAG-tagged (B and D) NS1 or NS2 expression vector or the empty vector SB897. Cells were treated with vehicle or 100 nM dexamethasone for 5 h. Firefly luciferase was normalized to renilla luciferase and fold increase following dexamethasone treatment was calculated. Means and SD are shown (n= 3). Significance was tested using a 1-way ANOVA and Dunnett post hoc test. a; p = 0.05-0.01 when compared to 5 ng SB897: b; p = 0.05-0.01 when compared to 10 ng SB897; c; p < 0.001 when compared to 5 ng SB897; d; p < 0.001 when compared to 10 ng SB897. Cell lysates were taken for western blotting using antibodies directed against the HA- (C) or FLAG-tags (D). The blots were stripped and re-probed for β-actin. A representative figure of two repeats is shown.