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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2013 Nov;57:73–82.

Figure 8. pGSN protects against gp120-induced neuronal damage via Kv2.1.

Figure 8

Neurons were exposed to gp120 (500pM), pGSN (2.5µg/ml), gp120(500pM) and pGSN (2.5µg/ml), or heat-inactivated pGSN (2.5µg/ml) in the presence or absence co-incubated GxTx (12.5 or 25 nM) for 24h. (A) Cell viability determined by MTT assay exhibited that gp120 exposure reduced cell viability (p<0.01 compare to Control), which was attenuated by GSN or GxTx. Heat-inactivated pGSN (§) showed no protective effect. pGSN or GxTx alone did not alter cell viability. (B) left panel shows TUNEL staining results as a measure of apoptosis, with cell nuclei visualized using Dapi stain. 10 visual fields were analyzed for each of three independent experiments. Gp120 induced neuronal apoptosis that was attenuated by either pGSN or GxTx. These data suggest that pGSN protects against gp120-induced neuronal damage via Kv2.1. **p<0.01 vs. control; #p<0.05 vs. gp120 group; ## p<0.01 vs. gp120 group. Scale bars denote 50µm.