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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Nutr Biochem. 2013 Dec;24(12):2051–2063. doi: 10.1016/j.jnutbio.2013.07.006

Fig. 1.

Fig. 1

(A) RT-PCR analysis of liver CYP1A1 mRNA expression in ApcMin-exposed mice. Mice were exposed to either vehicles (TC, USF or SF) or B(a)P (50 μg TC, 100 μg TC, 50 μg USF, 100 μg USF, 50 μg SF and 100 μg SF) for 60 days via oral gavage. The relative expression of CYP1A1 was quantified by densitometric quantitation of CYP1A1 bands and normalized to level of 18 sRNA. The bars represent mean±S.D. for three independent experiments (n = 7 animals for this and all other assays unless otherwise mentioned). *P<.005, when B(a)P+TC is compared to B(a)P+USF and B(a)P+SF; and when B(a)P+USF is compared to B(a)P+SF; 100 μg B(a)P+TC and 50 μg B(a)P+SF are compared to 50 μg B(a)P+TC and 100 μg B(a)P+SF, respectively; and when SF control is compared to 50 μg B(a)P+SF and 100 μg B(a)P+SF. (B) Western blot analysis of liver CYP1A1 protein expression in ApcMin-exposed mice. The relative expression of CYP1A1 was quantified by densitometric quantitation of CYP1A1 bands and GAPDH was used as the loading control. The bars represent mean±S.D. for three independent experiments. *P<.005, when B(a)P+SF is compared to other B(a)P treatment groups. (C) Enzyme activity of liver CYP1A1 in ApcMin-exposed mice. The activity of CYP1A1 was determined using luminescence detection. The bars represent mean±S.D. for three independent experiments.