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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Nutr Biochem. 2013 Dec;24(12):2051–2063. doi: 10.1016/j.jnutbio.2013.07.006

Fig. 5.

Fig. 5

(A) RT-PCR of colon CYP1B1 mRNA expression in ApcMin-exposed mice. The relative expression of CYP1B1 was quantified by densitometric quantitation of GST bands and normalized to level of 18 sRNA. The bars represent mean±S.D. for three independent experiments. *P<.005, when the 100 μg B(a)P+TC is compared to 50 μg B(a)P+TC; and when 50 μg B(a)P+USF and 50 μg B(a)P+SF are compared to 100 μg B(a)P+USF and 100 μg B(a)P+SF, respectively. (B) Western blot of colon CYP1B1 protein expression in ApcMin-exposed mice. The relative expression of CYP1B1 was quantified by densitometric quantitation of CYP1B1 bands and GAPDH was used as the loading control. The bars represent mean±S.D. for three independent experiments. *P<.005, when 50 μg B(a)P+USF and 100 μg B(a)P+USF are compared to 50 μg B(a)P+TC and 100 μg B(a)P+TC, respectively. (C) Enzyme activity of colon CYP1B1 in ApcMin-exposed mice. The activity of CYP1B1 was determined using luminescence detection. The bars represent mean±S.D. for three independent experiments. *P<.005, when B(a)P+TC compared to TC control; 50 μg B(a)P+TC and 100 μg B(a)P+TC are compared to 50 μg B(a)P+SF and 100 μg B(a)P+SF, respectively; and when 50 and 100 μg B(a)P+USF are compared to their respective B(a)P+SF.