Table 1. Bacterial strains and plasmids used in this study.
A. baumannii Strain | Relevant Genotype or Phenotype 1 | Source or Reference 2 |
ATCC 17978 | Cerebrospinal fluid | ATCC |
98-37-09 | Cerebrospinal fluid | [17] |
ACJ7 | EZ-Tn5::A1S_3026 derivative of 98-37-09 | This study |
E. coli strain | Relevant Genotype or Phenotype | Source or Reference |
INVαF′ | Competent cells | Invitrogen |
BL21 (DE3) | Competent cells | Invitrogen |
Plasmids | Relevant Genotype | Source or Reference |
pCR®II-TOPO® | Ampr Kanr | Invitrogen |
pWH1266 | Ampr Tetr | [34] |
pET30-LIC | Kanr | Novagen |
pACJ02 | pWH1266/A1S_3026, Tetr | This study |
Ampicillin (Amp); Kanamycin (Kan); Tetracycline (Tet), Resistant (r).
American Type Culture Collection (ATCC).