Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 28;9(1):e86143. doi: 10.1371/journal.pone.0086143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Yang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(a) Representative microscopic appearance of MCA-induced foci. BALB/c 3T3 cells were treated with 5 μg/ml MCA (MCA5) and vehicle (MCA0) separately, and cultured for 14 (14d) and 35 days (35d); scale bar = 500 μm. (b) Cell morphology of individual cells of untreated BALB/c 3T3 cells (control) and MCA-treated BALB/c 3T3 cells (MCA5/35d); scale bar = 50 μm. (c) Alteration of morphological phenotypes and cytoskeleton organization of BALB/c 3T3 cells treated by different MCA doses (0.1, 1, 2, 5 μg/ml) and cultured periods (14, 21, 28, 35 days); smooth muscle actin (red), DAPI (blue), scale bar = 50 μm. (d) Cells in the control and MCA-treated groups were analyzed using flow cytometry. The forward scatter (FSC) and side scatter (SSC) of particles were simultaneously measured. All experiments had been repeated at least three times.