Table 2. Analysis of variance of lag phase, viability change, and SSU1 expression according to the translocation and total SO2 concentration in the medium.
Lag phase (h) a | LP Viability a | SSU1/ACT1 b | ||||
part of variance | p value | part of variance | p value | part of variance | p value | |
Translocation | 36.4 | <0.001 | 36.2 | <0.001 | 28.4 | <0,001 |
SO2 | 22.2 | <0.001 | 8.0 | <0.05 | 0.5 | ns |
Translocation:SO2 | 34.9 | <0.001 | 26.4 | <0.01 | 2.3 | ns |
qPCR block | nd | nd | 8.0 | <0.05 | ||
Residual | 6.4 | 29.4 | 60.8 |
the model used was Z1 = μ+translocation (i,j,k)*SO2(i,j,k) +ε i, which allows the estimation of the part of the variance explained by the chromosome XVI forms (n = 3) and the SO2 concentration (n = 3). For each condition, 3 repetitions were analyzed. nd = not determined.
the model used was Z2 = μ+translocation (i,j,k)*SO2(i,j,k)+qPCR(i,j) +ε i, which allows the estimation of the part of the variance explained by the chromosome XVI forms (n = 3), the SO2 concentration (n = 3), and the qPCR blocks. For this model 6 to 9 repetitions were analyzed for each conditions depending on the lag phase time. ns = not significant.