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. 2014 Jan 28;9(1):e86298. doi: 10.1371/journal.pone.0086298

Table 2. Analysis of variance of lag phase, viability change, and SSU1 expression according to the translocation and total SO2 concentration in the medium.

Lag phase (h) a LP Viability a SSU1/ACT1 b
part of variance p value part of variance p value part of variance p value
Translocation 36.4 <0.001 36.2 <0.001 28.4 <0,001
SO2 22.2 <0.001 8.0 <0.05 0.5 ns
Translocation:SO2 34.9 <0.001 26.4 <0.01 2.3 ns
qPCR block nd nd 8.0 <0.05
Residual 6.4 29.4 60.8
a

the model used was Z1 = μ+translocation (i,j,k)*SO2(i,j,k) i, which allows the estimation of the part of the variance explained by the chromosome XVI forms (n = 3) and the SO2 concentration (n = 3). For each condition, 3 repetitions were analyzed. nd = not determined.

b

the model used was Z2 = μ+translocation (i,j,k)*SO2(i,j,k)+qPCR(i,j) i, which allows the estimation of the part of the variance explained by the chromosome XVI forms (n = 3), the SO2 concentration (n = 3), and the qPCR blocks. For this model 6 to 9 repetitions were analyzed for each conditions depending on the lag phase time. ns = not significant.