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Histochemical analysis of chondrocyte-specific phenotypes induced by herbal extracts. ATDC5 cells were seeded and allowed to differentiate. Thereafter, cells were cultured in the absence (Control) or presence of 10 μM AD, or 1, 10 and 100 μg/ml of C. atratum, M. azedarach and C. turtschaninovii extracts. After 3 days, cells were subjected to crystal violet (CV), ALPase activity (ALP) and toluidine blue (TB) staining, and examined under a microscope. Bar, 100 μm.
