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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Cell Rep. 2013 Dec 12;5(6):1600–1610. doi: 10.1016/j.celrep.2013.11.018

Figure 6. Cell-non-autonomous activation of DAF-16 by rsks-1 RNAi knocking-down in the germ line.

Figure 6

(A) Activation of the DAF-16 target stdh-1 by rsks-1 RNAi in daf-2 and daf- 2; rrf-1. rsks-1 RNAi knocking-down in the germ line of daf-2 animals (daf-2; rrf-1) significantly increased the intestinal expression of GFP driven by the stdh-1 promoter. Arrows indicate the anterior and posterior intestine. (B) Quantification of GFP expression driven by the stdh-1 promoter. Thirty animals were examined for each treatment. (C) Measurement of Pstdh-1::gfp expression by Western blots using anti-GFP antibodies. (D) Quantification of GFP expression from Western blots. Relative GFP levels were calculated by normalizing the intensities of GFP bands to Actin. Fold increase in Pstdh-1::gfp expression induced by rsks-1 RNAi was calculated through dividing the relative GFP levels in rsks-1 RNAi-treated animals by those in control RNAi-treated animals. **, p < 0.01. The quantification was performed with four biological replicates.