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. 2014 Jan 28;9(1):e86983. doi: 10.1371/journal.pone.0086983

Table 2. Mean energy transfer efficiency values (ETeff) at pH 7.4 and pH 3.5.

αS βS γS
Construct pH 7.4 pH 3.5 pH 7.4 pH 3.5 pH 7.4 pH 3.5
AH 0.84±0 0.88±0.01+ 0.83±0.01 0.89±0*;+ 0.87±0.01* 0.90±0.01*;+
LF 0.88±0.1 0.88±0.02 0.87±0.01 0.91±0.01*;+ 0.84±0.01* 0.87±0+
NAC 0.82±0 0.89±0.01+ 0.87±0* 0.90±0.01+ 0.78±0.01* 0.84±0.01*;+
CT 0.84±0.01 0.91±0.01+ 0.71±0.01* 0.92±0+ 0.79±0.01* 0.88±0.01*;+

ETeff values corresponding to the mean energy transfer efficiency obtained from histograms as described in the Materials and Methods (n = 4 for all constructs except for αS LF pH 7.4 where n = 3); error is standard deviation of the mean. Significance level of βS or γS ETeff values relative to αS (*); significance level in ETeff for each protein at pH 3.5 relative to pH 7.4 (+). */+ indicates results that are significant at the 5% level using a two-tailed Mann-Whitney U-test. AH–amphipathic helix motif-containing construct; LF–flexible loop forming construct; NAC–non-amyloid beta component or hydrophobic core construct; CT–C-terminal construct.