Table 2. Mean energy transfer efficiency values (ETeff) at pH 7.4 and pH 3.5.
αS | βS | γS | |||||
Construct | pH 7.4 | pH 3.5 | pH 7.4 | pH 3.5 | pH 7.4 | pH 3.5 | |
AH | 0.84±0 | 0.88±0.01+ | 0.83±0.01 | 0.89±0*;+ | 0.87±0.01* | 0.90±0.01*;+ | |
LF | 0.88±0.1 | 0.88±0.02 | 0.87±0.01 | 0.91±0.01*;+ | 0.84±0.01* | 0.87±0+ | |
NAC | 0.82±0 | 0.89±0.01+ | 0.87±0* | 0.90±0.01+ | 0.78±0.01* | 0.84±0.01*;+ | |
CT | 0.84±0.01 | 0.91±0.01+ | 0.71±0.01* | 0.92±0+ | 0.79±0.01* | 0.88±0.01*;+ |
ETeff values corresponding to the mean energy transfer efficiency obtained from histograms as described in the Materials and Methods (n = 4 for all constructs except for αS LF pH 7.4 where n = 3); error is standard deviation of the mean. Significance level of βS or γS ETeff values relative to αS (*); significance level in ETeff for each protein at pH 3.5 relative to pH 7.4 (+). */+ indicates results that are significant at the 5% level using a two-tailed Mann-Whitney U-test. AH–amphipathic helix motif-containing construct; LF–flexible loop forming construct; NAC–non-amyloid beta component or hydrophobic core construct; CT–C-terminal construct.