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. 2014 Jan 28;9(1):e87076. doi: 10.1371/journal.pone.0087076

Figure 1. Design of oligonucleotides for dRT-PCR for detection of HPAI virus sequences in H7 subtype viruses.

Figure 1

The sequences for (A) the common forward primer, (B) the common reverse primer, (Probe 1) a common probe for quantification of both HPAI and LPAI H7 virus HA amplicons and (Probe 2) a probe to detect the specific HPAI virus amplicon are shown. The sequences corresponding to the virus HA genes are from LPAI and HPAI viruses isolated during the H7N1 avian influenza virus outbreak in turkeys, 1999–2000. Numbering refers to the HPAI virus nucleotide sequence beginning from HA gene start codon.