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. 2014 Jan 28;9(1):e87256. doi: 10.1371/journal.pone.0087256

Figure 1. Association of monomers, changes in polarity inside the aggregates.

Figure 1

a, The efficiency of fluorescence energy transfer between 0.5 µM dansyl-labeled HEWL (donor) mixed with 0.5 µM dabcyl-labeled HEWL (acceptor) in presence of excess unlabeled HEWL at different time intervals post-incubation for 3, 50 & 120 µM HEWL in pH 12.2 and 50 µM in pH 7.0 are shown. For 0.3 µM, a 10 fold dilution of 3 µM sample was employed. The data were fitted by an equation identical to Eq. 2 after replacing anisotropy with FRET efficiency. b, Changes in emission λmax of dansyl-labeled HEWL (alone) as above. c, Changes in emission λmax, and d, Integrated normalized emission intensity of ANS (10 µM) in presence of HEWL are shown for different time intervals post-incubation of HEWL at concentrations 0.3, 3, 10, 50, & 120 µM in pH 12.2 and 3 µM in pH 7. For all data in panel, filled symbols indicate pH 7, while unfilled symbols represent pH 12.2. Each HEWL concentration is represented by a unique symbol. Error bars, SD; N  =  3.