Skip to main content
. 2014 Jan 28;9(1):e87474. doi: 10.1371/journal.pone.0087474

Figure 6. The effects of IGF-1 and PPP on IGF-1R autophosphorylation and on its downstream signalling proteins and cell cycle proteins in L428 cells.

Figure 6

A, pIGF-1R level was measured at different time points after stimulation with IGF-1 (50 ng/mL). Phosphorylation levels of IGF-1R, Akt and ERK were increased upon IGF-1 stimulation at 20 and 30 minutes. B, phosphorylation of IGF-1R, and Akt upon IGF-1 stimulation could be blocked by the pIGF-1R kinase inhibitor PPP in L428 cells. While phosphorylation of ERK was increased by PPP (C) PPP treatment induced a reduction of Cdc2 phosphorylation and upregulation of CyclinB1, consistent with the occurrence of a G2/M-phase cell cycle arrest.