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. 2014 Jan 28;9(1):e87475. doi: 10.1371/journal.pone.0087475

Figure 2. Pyrosequencing analysis of 4 tumour suppressor genes following DAC treatment.

Figure 2

Changes in methylation status of a CpG site for 4 representative candidate genes (CSMD1, RARB, CADM1 and CDH13), following treatment with DAC at a range of concentrations in 8 primary AML patient cultures. Cells were treated at 0, 24, 48, 72 and 96 h, DNA methylation was measured at 120 h. All experiments were performed in duplicate.