Figure 1.

Alteration of glucose levels alters cell proliferation in a panel of human breast cancer cell lines. (A) The indicated human breast cancer cell lines were treated with various glucose concentrations (0, 5, 7, 10, 17 mmol/L) for 72 h then assayed for percent proliferation using MTS and normalized to 5 mmol/L glucose. (B–D) Indicated breast cancer cell lines used above were seeded in media containing 5 mmol/L (B), 10 mmol/L (C), or 17 mmol/L (D) glucose and treated with vehicle control, 5 mmol/L or 10 mmol/L metformin for 72 h. The percent cell proliferation was measured by MTS assay and normalized to control treatment. Experiments are representative of triplicate assays. Data are representative of averages of triplicate determinations ± SE.