Figure 2.

Reduction of glucose enhances metformin-mediated inhibition of colony formation and motility in TNBC, as compared with Luminal A or HER2 overexpressing cell lines. (A) Luminal A (T-47D), HER2 overexpressing (SK-BR-3), or TNBC (MDA-MB-468, MDA-MB-231) cell lines were treated with 5 mmol/L metformin in media containing various glucose levels (5, 10, 17 mmol/L) as described. (B) Enumeration of colony forming units for each cell line was performed see “Materials and Methods”. Bar graphs show averages of 3 independent experiments ± SE.