Figure 5. Effect of YB-1 cleavage on its DNA binding properties and gene expression regulation. (A and B) YB-1 binding to nonspecific [³²P]-labeled 660-nt DNA duplex (A) or 18-nt Y-box-containing ssDNA (B) was analyzed by EMSA (left panels) and filter binding assay (right panels), as described in “Materials and Methods”. (C) Venn diagram showing common and differentially expressed RNA subsets identified by microarray analysis in NIH3T3 cells expressing WT or truncated YB-1 proteins. (D) Functional categories of genes whose expression was changed in NIH3T3 cells expressing WT or truncated YB-1 proteins, as compared with vector alone cells. Analysis was done using DAVID Bioinformatics Resources (http://david.abcc.ncifcrf.gov).