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Figure 6. RT-PCR analysis of transcripts of pluripotent genes in rapamycin-derived cells.RNA was prepared from REFs (passage 3), rapamycin-derived cells (passage 21), and mESCs (positive control). RT-PCR step was performed by using primers to Oct4, Sox2, Nanog, and Tert genes. Gapdh mRNA level was taken as internal control.
